Great Britain's Heather Watson and Laura Robson were knocked out in the first round of the Nottingham Open.
British number two Watson, 24, took the first set against Magdalena Rybarikova but went down 4-6 6-0 6-4.
Robson, 22, had her serve broken six times as she lost 6-3 7-5 to Portugal's Michelle Larcher de Brito.
Naomi Broady bowed out after a 6-2 6-1 loss to Su-Wei Hsieh, but British number four Tara Moore progressed by beating Croatia's Donna Vekic 6-2 7-5.
The 23-year-old joined British number one Johanna Konta in the second round, after Konta triumphed 6-3 6-0 over American Victoria Duval on Monday to set up a tie with China's Zheng Saisai.
Moore will play American eighth seed Christina McHale on Thursday, having won only her second match in the main draw of a WTA event.
The world number 280 won an ITF event in Eastbourne last week, but may need a wildcard to play at Wimbledon.
"I'm still in the tournament here so I am just going to look forward to the next match I'm playing," she said.
"As far as the next few weeks go, I'm not sure yet. I have put in a request but it's not my decision. It's the All England Club's decision and I respect the decision."
Former world number one Caroline Wozniacki also reached the second round with a 7-5 6-3 win over Cagla Buyukakcay, but fifth seed Yanina Wickmayer was defeated 7-5 7-6 by 'lucky loser' Andrea Hlavackova.
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