Naomi Broady's Wimbledon challenge ended in the second round as she lost 6-3 6-2 to former world number one Caroline Wozniacki on Court One.
British number four Broady earned her first win at a Grand Slam by defeating Hungary's Timea Babos on Monday.
But Denmark's Wozinacki, seeded 16th, beat the 24-year-old in 59 minutes.
Earlier, British number six Tara Moore lost 6-4 6-7 (3-7) 9-7 to Russian Vera Zvonareva in a delayed finish to their first-round match.
|Virginia Wade on Broady's defeat|
|"When you have as much power as Naomi Broady has, it's tempting to go for big shots when you'd be better off being more cautious. But she certainly got into the spirit of things. She's enjoyed it, and she's not hanging her head by any means."|
Wildcard Broady, from Stockport, has paid her own way on the circuit since the Lawn Tennis Association (LTA) cut her funding in 2007 - when she was 17 - for "unprofessional behaviour" and "lack of discipline".
She went 3-0 down against Wozniacki after losing her opening service game, but refused to buckle and saved two set points before sending a forehand wide as the Dane took the opening set in half-an-hour.
Broady's inconsistent forehand held back her attempts to get back into the match, and her hopes were dented further when she was broken in the opening game of the second set.
Wozniacki secured a second break of serve to lead 4-1, although Broady won support from the crowd for a spirited response, winning her next service game to love to stay in the match.
The Dane was ultimately too strong, and set up a third-round meeting with Croatia's Ana Konjuh with a fierce serve that Broady looped long with an attempted return.
Moore, meanwhile, was left frustrated after a chance of victory against Zvonareva slipped away.
Bad light stopped play on Tuesday with Moore, 21, levelling the match with a strong performance in the tie-break.
Resuming on Wednesday, the decider was a tense affair, but Zvonareva broke in the 16th game to win the match.
|Sam Smith on Moore's defeat|
|"Tara Moore played such good tennis in this match but made one disastrous mistake at 5-3 serving for the match. She waited for Zvonareva to make the mistakes - and they never came. It is a shame because she is good enough to be in that second round."|
Moore twice led with a break in the deciding set and served for the match at 5-4 as 2010 finalist Zvonareva saw her unforced error count rise dramatically - she hit 44 to Moore's 29.
But former world number two Zvonareva, who has dropped to 566 in the rankings after 18 months out with a shoulder injury, broke back to level the match.
The next five games went with serve and though Moore saved two match points on her serve, she could do nothing about the third as Zvonareva fired a backhand winner down the line to win in two hours and 43 minutes.
Moore told BBC Sport: "The overwhelming feeling is one of disappointment. I gave all I had out there, I fought really hard.
"Vera is very good and played well in the tough moments. She just had too much for me out there sometimes."
Elsewhere, Britain's Jamie Murray and Australian John Peers, the 14th seeds, beat Britain's Dan Evans and James Ward 6-2 6-4 6-4 in the men's doubles.