Colin Fleming and Ross Hutchins were knocked out of the Australian Open doubles with a third-round defeat by American top seeds Bob and Mike Bryan.
The Brits responded to losing the first set by outclassing the twins in the second, but went down 6-4 0-6 6-2.
In the juniors, Liam Broady knocked out Italy's Matteo Donati, Kyle Edmund beat Alexandre Favrot of France and Luke Bambridge overcame German Daniel Masur.
Joshua Ward-Hibbert saw off Australia's Matthew Tanza, losing just three games.
Wimbledon boy's singles runner-up Broady, 18, is the third seed, while Edmund is seventh and fellow 17-year-olds Bambridge and Ward-Hibbert are unseeded.
Next up for Broady is Robin Stanek of the Czech Republic, Edmund faces Spaniard Pol Toledo Bague, Bambridge goes up against Japanese fourth seed Kaichi Uchida and Ward-Hibbert plays eighth seed Stefano Napolitano.
Fleming and Hutchins will now turn their attention to Great Britain's Davis Cup tie against Slovakia in Scotland on 10-12 February.
Fleming felt their display gave cause for optimism, telling BBC Radio 5 live: "The world's top eight is a huge goal.
"We will take a lot of positives from this, but now we have to look at how they managed to steal momentum at the start of the third set."
Hutchins added: "It was a decent display out there, especially in the second set, but we're of the belief that we're at a level to match the best teams in the world.
"We're good enough to win these matches. We have to learn how we can improve to go further next time and win a Grand Slam.
"That is what we want to do. It comes with experience and by working harder."