Wigan forward Sean O'Loughlin has been ruled out of England's International Origin match against the Exiles on Friday because of a knee injury.
O'Loughlin, 30, missed Wigan's 33-32 win against Super League rivals Widnes last Friday with the same problem.
"His recovery has been slower than expected," said England's chief medical officer Dr Chris Brookes.
England coach Steve McNamara will not call up a replacement for the mid-season international in Warrington.
"The Wigan medical staff will be liaising with the England medical team about his future progress," added Brookes.
O'Loughlin's withdrawal is the latest setback for McNamara, who is already without Leeds Rhinos trio Carl Ablett, Danny McGuire and centre Kallum Watkins, plus Wigan hooker Michael McIllorum and Hull FC second-row Danny Tickle.
The Exiles, coached by ex-Rhinos boss Brian McClennan, are made up of foreign Super League players.
ENGLAND SQUAD TO FACE EXILES
Rob Burrow (Leeds Rhinos), Josh Charnley (Wigan Warriors), Rangi Chase (Castleford Tigers), Eorl Crabtree (Huddersfield Giants), Leroy Cudjoe (Huddersfield Giants), Gareth Ellis (Hull FC), Liam Farrell (Wigan Warriors), Brett Ferres (Huddersfield Giants), Ryan Hall (Leeds Rhinos), Zak Hardaker (Leeds Rhinos), Chris Hill (Warrington Wolves), Jamie Jones-Buchanan (Leeds Rhinos), Adrian Morley (Warrington Wolves), Lee Mossop (Wigan Warriors), Richie Myler (Warrington Wolves), James Roby (St Helens), Kevin Sinfield (Leeds Rhinos), Sam Tomkins (Wigan Warriors), Ben Westwood (Warrington Wolves)