The Dutch rowing team have joined Great Britain in withdrawing from next weekend's Hamburg World Cup regatta.
The news comes after Fisa, the international rowing federation, said the event poses "no particular risk for participants" on Thursday.
Great Britain's rowing team pulled out of the event on Wednesday due to fears over the E. coli outbreak in Germany.
The Dutch team announced their withdrawal on their website later on Thursday.
Great Britain performance director David Tanner took the decision after "a risk-based assessment relating to the continuing issue of E. coli in the area."
Fisa conducted its own risk analysis in conjunction with the local Hamburg government and concluded there "is no particular risk for participants".
The German Rowing Association, which is organising the regatta, said in a statement on its website that it feels "there is no reason to cancel any big events in Hamburg", highlighting the successful running of a "top-class equestrian tournament with international presence as well as the largest junior rowing regatta" in the city last weekend.
Following the withdrawal of the GB and Dutch teams, there are still 35 countries due to take part in the event which runs from 17-19 June.
Great Britain won 11 medals, including five golds, at the Munich World Cup regatta at the end of May, but missing Hamburg will effectively end their chances of winning the overall title.
The move could open the way for more of the GB team to compete at Henley Royal Regatta, which starts on 29 June, and is a week before the third and final World Cup regatta in Lucerne, Switzerland.
The World Championships are scheduled to take place in Bled, Slovenia at the end of August.