Kevin Manning will ride Godolphin's Sudden Wonder in Saturday's Derby at Epsom as further jockey bookings were finalised on Tuesday.
The Irish jockey, who won the 2008 Derby on New Approach, replaces Mickael Barzalona.
Apprentice jockey Oisin Murphy, 18, will have his first Derby ride after being booked for Red Galileo.
Meanwhile, William Buick has chosen to partner Sandown Classic Trial winner Western Hymn over stablemate Romsdal.
Barzalona rode Sudden Wonder in its last four races, but trainer Charlie Appleby believes Manning's Epsom experience makes him most suited for the ride.
"I have spoken to connections and we agreed that we would like Kevin Manning to be on board as his style of riding will suit Sudden Wonder," Appleby said.
"He is a very positive rider and experienced around Epsom. He partnered New Approach, Sudden Wonder's sire, to victory in the Derby."
Rising star Murphy impressed trainer Ed Dunlop when he secured his first Group-race win by riding Hot Streak to Temple Stakes glory at Haydock last month.
"We had a list of possible jockeys and after watching him win the Temple Stakes, the decision was made to book Oisin," said Dunlop.
Murphy will also ride William Knight-trained Beacon Lady in the Coronation Cup on the day.
Derby entries at confirmation stage: Impulsive Moment (trainer, A Balding), Arod (P Chapple-Hyam), Australia (A P O'Brien), Ebanoran (John Oxx), Geoffrey Chaucer (A P O'Brien), Kingfisher (A P O'Brien), Orchestra (A P O'Brien), Pinzolo (C Appleby), Snow Sky (Sir M Stoute), Sudden Wonder (C Appleby), True Story (S Bin Suroor), Western Hymn (J Gosden), Fascinating Rock (D K Weld), Kingston Hill (R Varian), Red Galileo (E Dunlop), Our Channel (W Haggas), Romsdal (J Gosden)