Spain's Sergio Garcia survived a seven-man play-off to secure his place in this month's US Open.
Garcia, who has dropped to 75 in the world rankings, said last month that he would not go through qualifying to continue his run of 47 majors in a row.
However, he shot rounds of 68 and 67 in Memphis, Tennessee to claim one of the four spots available in the play-off.
But Vijay Singh will miss the event at Congressional Country Club in Maryland from 16-19 June.
Singh opted not to take part in qualifying and his run of 67 consecutive majors, which goes back to the 1994 US Open at Oakmont, is over.
His only other chance of getting into the major was via a top finish in this week's St Jude Classic, but the PGA website said he was no longer in that field.
Among the others who earned places at the US Open were Sam Saunders, the grandson of former winner Arnold Palmer; Steve Irwin, the son of three-time champion Hale Irwin; and college freshman Patrick Cantlay.
But previous champions Steve Jones, Lee Janzen and Tom Kite missed out, along with former Open winners Justin Leonard, David Duval and Ben Curtis.