Match ends, Ayr United 1, Queen of the South 1.
Queen of the South's Connor Murray scored with his first touch to deny Ayr United a fourth consecutive win.
Michael Rose's header from Alan Forrest's corner had put the Honest Men in front a few minutes earlier.
Queens' captain Stephen Dobbie hit the inside of the post in a goal-less first half.
Lawrence Shankland and Craig Moore had a couple of half-chances for United but visiting goalkeeper Alan Martin dealt with those attempts.
The draw means Ayr's lead at the top of the Championship is increased to four points, given Ross county lost to Dundee United.
But what has gone is the hosts perfect record at Somerset Park this season.
Ayr United manager Ian McCall: "I'd love to see the possession stats, it must be between 60% and 70% to us. We were really dominant.
"I thought Stephen Dobbie had his boots on the wrong feet in the first half and then he nearly scores an absolute wonder goal. But we should have been 2-0 or 3-0 up and the game should have been put to bed."
Queen of the South manager Gary Naysmith: "Ayr have won all their games here, so for us to take a point and we've now taken four points from six from them - we've got to be pleased.
"I tried to play a more attacking formation right from the off and it didn't really work. It caused us problems deeper in the pitch. Young Connor Murray comes off the bench for the equaliser and that'll do him the world of good."
- 10ForrestSubstituted forMcGuffieat 76'minutes
- 4KerrSubstituted forMcDaidat 82'minutes
- 18MurdochBooked at 80mins
- 9MooreSubstituted forMoffatat 80'minutes
Queen of Sth
- 10ToddSubstituted forStirlingat 65'minutes
- 22NormanSubstituted forMurrayat 81'minutes
- 14HarkinsSubstituted forBellat 90+3'minutes
- John Beaton
- Shots on Target
Second Half ends, Ayr United 1, Queen of the South 1.
Attempt saved. Steven Bell (Ayr United) header from the centre of the box is saved in the top right corner.
Substitution, Queen of the South. Owen Bell replaces Gary Harkins.
Liam Smith (Ayr United) wins a free kick on the left wing.
Foul by Gary Harkins (Queen of the South).
Attempt saved. Lawrence Shankland (Ayr United) right footed shot from outside the box is saved in the top centre of the goal.
Attempt missed. Craig McGuffie (Ayr United) left footed shot from outside the box is close, but misses to the right.
Foul by Michael Rose (Ayr United).
Stephen Dobbie (Queen of the South) wins a free kick in the attacking half.
Lawrence Shankland (Ayr United) wins a free kick in the defensive half.
Foul by Callum Semple (Queen of the South).
Substitution, Ayr United. Declan McDaid replaces Mark Kerr.
Goal! Ayr United 1, Queen of the South 1. Connor Murray (Queen of the South) right footed shot from the right side of the box to the bottom left corner. Assisted by Callum Fordyce.
Substitution, Queen of the South. Connor Murray replaces David Norman.
Substitution, Ayr United. Michael Moffat replaces Craig Moore.
Andy Murdoch (Ayr United) is shown the yellow card for a bad foul.
Foul by Andy Murdoch (Ayr United).
David Norman (Queen of the South) wins a free kick in the attacking half.
Substitution, Ayr United. Craig McGuffie replaces Alan Forrest.