Reading manager Jaap Stam warned his players there is "no chance of relaxing" in the Championship after their defeat by Brentford.
The Royals' six-match unbeaten league run was ended in a 4-1 reverse at Griffin Park on Tuesday.
"We've had a good start and done well up until now," Stam told BBC Radio Berkshire. "You want to learn from every game you play.
"Sometimes when you don't start a game well, the opponent can take advantage."
Reading were 3-0 behind to Brentford after an hour and despite Yann Kermorgant's second-half penalty, were easily second-best.
"You need to know that if you want to win games, you need to be sharp as a team in what you want to do," Stam added.
"There's no chance for relaxing or thinking we're going to do this at 80%. Every game we need to give the full 100% and we need to be fully focused.
"But, you don't need to go back to square one after this. We've had a good run and we want to keep that up."
Reading, who slipped out of the top six following the defeat, face Derby at home on Saturday.