Partick Thistle's Steven Lawless has been accused of breaching Scottish Football Association rules by allegedly betting on 513 games.
It is claimed the 24-year-old midfielder placed the bets between December 2012 and March 2015.
He has been called to appear before an SFA-appointed judicial panel on Thursday, 21 May.
The SFA notice of complaint stresses there is no evidence of Lawless betting on games involving his own team.
SFA disciplinary rule 33 prohibits players, coaches, club officials and referees in Scotland from betting on football anywhere in the world.
Earlier this season, Rangers keeper Steve Simonsen was banned for two games after being found guilty of betting on 55 games.
Lawless played in last weekend's 1-1 draw at Hamilton, two days after the Glasgow club announced that they were conducting their own internal investigation into the gambling allegations.