Match ends, Brighton and Hove Albion 1, Barnsley 2.
Barnsley remained at the foot of the Championship despite their first away win of the season at Brighton.
The victory came in their first game under caretaker manager Micky Mellon following the sacking of David Flitcroft at the weekend.
A fine individual effort from Paddy McCourt gave the Tykes a first-half lead, which was doubled five minutes after the break by Jacob Mellis.
Matthew Upson's header halved the deficit, but Barnsley held out.
Wins for Yeovil and Sheffield Wednesday meant the Yorkshire club remain at the bottom of the table on goal difference.
The hosts started well, with Barnsley keeper Jack Butland forced into a fine save when he tipped Craig Conway's shot onto the crossbar.
But Barnsley ended a sequence of nearly five hours without a goal when McCourt ended a solo run with a one-two with Marcus Tudgay before slotting home.
Tudgay was also involved in the second goal, threading the ball to Mellis inside the area before the midfielder beat keeper Tomasz Kuszczak.
Upson pulled a goal back with a header from a corner, and referee James Linington was forced off with an injury, but Barnsley held out to end a run of six games without a win.
Brighton manager Oscar Garcia: "We only played 55 minutes in this game. But we did get a reaction in the second half and had chances for further goals.
"The problem was we started very slowly. We wanted to start well, but Barnsley kept the ball pretty well, and if we don't have the ball we're not comfortable.
"It's a tough league and there are no easy games, all the players are really disappointed at how we played."
Barnsley caretaker manager Micky Mellon: "I've been friends for life with David (Flitcroft) and I know what it's like to be sacked. It was a tough night last Saturday but it was done very professionally.
"We knew we had to be really professional at Brighton and the key was that I wanted us to keep the ball.
"I was delighted with both the goals and a lot of the time we were in total control. We were determined to pass it and be brave and we achieved that."
- 18Forster-CaskeySubstituted forBuckleyat 45'minutes
- 9BarnesSubstituted forLitaat 82'minutes
- 21López MorenoSubstituted forAgustienat 65'minutes
- 14WisemanBooked at 82mins
- 11PerkinsBooked at 53mins
- 3RamageBooked at 30mins
- 42McCourtSubstituted forDawsonat 75'minutes
- 23EtuhuSubstituted forScotlandat 90+2'minutesBooked at 90mins
- 21MellisBooked at 60minsSubstituted forO'Brienat 78'minutes
- James Linington
- Shots on Target
Second Half ends, Brighton and Hove Albion 1, Barnsley 2.
Attempt missed. Gordon Greer (Brighton and Hove Albion) header from the centre of the box is close, but misses to the right. Assisted by Craig Conway with a cross following a set piece situation.
Jason Scotland (Barnsley) is shown the yellow card for a bad foul.
William Buckley (Brighton and Hove Albion) wins a free kick on the right wing.
Foul by Jason Scotland (Barnsley).
Leroy Lita (Brighton and Hove Albion) wins a free kick on the right wing.
Foul by Martin Cranie (Barnsley).
Foul by Kemy Agustien (Brighton and Hove Albion).
Stephen Dawson (Barnsley) wins a free kick in the attacking half.
Foul by Liam Bridcutt (Brighton and Hove Albion).
Tomasz Cywka (Barnsley) wins a free kick in the defensive half.
Corner, Brighton and Hove Albion. Conceded by Martin Cranie.
Substitution, Barnsley. Jason Scotland replaces Kelvin Etuhu because of an injury.
Attempt missed. Craig Conway (Brighton and Hove Albion) right footed shot from outside the box is too high. Assisted by Liam Bridcutt.
Attempt saved. William Buckley (Brighton and Hove Albion) right footed shot from the centre of the box is saved in the top left corner. Assisted by Bruno.
Offside, Brighton and Hove Albion. Stephen Ward tries a through ball, but Leroy Lita is caught offside.
Attempt blocked. Leroy Lita (Brighton and Hove Albion) right footed shot from the centre of the box is blocked. Assisted by Stephen Ward with a cross.
Substitution, Brighton and Hove Albion. Leroy Lita replaces Ashley Barnes.
Scott Wiseman (Barnsley) is shown the yellow card for a bad foul.