Charlie Austin's 16th goal of the season was enough to give Burnley all three points against promotion-chasing Brighton.
The striker jinked his way through the Seagulls' defence to see his initial effort cleared, before reacting quickest to thunder in the rebound.
Ross Wallace could have doubled his side's advantage, hitting a long-range effort narrowly over the bar.
Clarets keeper Lee Grant denied the lively Craig Mackail-Smith late on.
The decisive moment came after 27 minutes when former Swindon forward Austin coolly converted Josh McQuoid's cross at the second attempt.
Danny Ings headed a teasing delivery from Kieran Trippier wide before Austin almost capitalised again when another cross from the former Manchester City youngster caused further havoc in the Seagulls' area.
Grant plunged to his left to make a superb save when Sam Vokes teed up Mackail-Smith two minutes after the restart.
Vicente also failed to convert after being hacked down by Michael Duff, scuffing his 25-yard free-kick into the bottom of the wall.
Ashley Barnes replaced Vokes after an hour and his first contribution was to blaze over from Joe Mattock's low, left-wing cross.
But Austin continued to press and speared a shot narrowly wide from McQuoid's knockdown, as the Clarets gained a well-earned victory.
Despite a remarkable run of just one Championship defeat in 2012, which has seen Brighton climb into the play-off places, they have now won only once in their last seven away games.
Meanwhile, Burnley's first home win since Valentine's Day keeps their play-off hopes alive.