Equatorial Guinea are already through to the last eight, but both sides will be looking to top Group A.
The co-hosts' captain Rodolfo Bodipo is out through injury, while defender Lawrence Doe is suspended after his red card in the dramatic win over Senegal.
Zambia have no injury worries and striker James Chamanga has recovered in time for the effective group decider.
Chipolopolo coach Herve Renard has indicated that he will stay loyal to his previous team selections.
The co-hosts are in the unique position of having the pressure taken off them ahead of the quarter-finals, after their heroic 2-1 win against Senegal on Wednesday and an opening victory over Libya.
They will, however, be looking to go one better and top Group A, which they will do with a draw against Zambia.
And National Lightning captain Juvenal Edjogo-Owono says he wants to top the group to avoid favourites Ivory Coast in the last eight.
"We are not afraid of anyone, but none of the players really want to play Ivory Coast at this stage," Edjogo-Owono said.
"All the supporters are saying they don't care who we play and that they're not worried about Ivory Coast.
"But we know Ivory Coast are a level above the rest."
The Chipolopolo can still head up the group with a win over the home side, but just need a draw to progress.
Renard has, meanwhile, raised the issue of the rules surrounding the naturalisation of players as they prepare for the clash with the co-hosts.
Equatorial Guinea's line-up against Libya and Senegal was made up of 11 players born outside the country - a mix of Spanish-based players, who qualify through their parents, and naturalised players from Brazil, Liberia, Cameroon and Cape Verde.
"I would be very disappointed if, in 10 years' time, I saw a national team fielding five players who didn't have any connection with that country," Renard said.
"I don't know if some [Equatorial Guinea players] are born here, if their parents were born here, and I don't want to know," he added.
"We will play against the Equatorial Guinea national team and we have to beat them, that's all."