Millwall Lionesses were relegated from the Women's Premier League following a 3-0 defeat by Reading, whose win took them up to second place.
Barnet were bottom of the table before the weekend matches but climbed out of the drop zone with a 1-0 win against champions Sunderland.
Watford slipped into the bottom two after a goalless draw at Leeds United.
The Super League launches on Thursday with home matches for Birmingham, Chelsea, Lincoln and Liverpool.
Millwall Lionesses 0-3 Reading
The Lionesses had to win to have any chance of retaining their place in the top flight, but were on the back foot after Nicky Watts opened the scoring in the 24th minute.
Fran Kirby doubled Reading's lead four minutes after the break, and there was no way back for the home side after Watts bagged her second goal in the 68th minute.
Barnet 1-0 Sunderland
Barnet have spent practically the whole of the season in the bottom two but eased their relegation fears thanks to a Vicky Boardman goal.
Boardman headed home Stacey Sowden 57th minute cross to break the deadlock in a hard-fought encounter.
The Black Cats responded with a series of late attacks and went close through Demi Stokes and Sophie Williams, but the Bees held out for a crucial victory.
Leeds United 0-0 Watford
Watford's hopes of maintaining their top-flight status took a knock as they failed to find the target in a tight contest.
It could have been worse for the Hornets as Leeds bossed the closing stages and went close to winning the match on a couple of occasions.
Emma Coates was particularly unlucky with an 80th-minute lob that bounced back into play off the underside of the bar.