Britain's Alex Dowsett overshadowed Sir Bradley Wiggins as he powered to a surprise win in the first individual time trial of the Giro d'Italia.
Dowsett, the British time trial champion, faced a long wait after setting the pace over the 54.8km course having started 39th of the 201 riders.
Wiggins recovered from a puncture to finish second, 10 seconds behind.
But Vincenzo Nibali was only 11 seconds further back to take the overall lead, with Wiggins now fourth, 1'16" adrift.
Essex-born Dowsett, riding in his first Grand Tour, completed the technically demanding route from Gabicce Mare to Saltara in one hour, 16 minutes, 27 seconds, with Wiggins second and Estonia's Tanel Kangert third.
"I feel like I got everything out," Dowsett told BBC Sport. "It was super difficult and there was no opportunity to get into a rhythm and I was just riding as hard as my legs would let me.
"It's different doing a time trial in a tour because you are fatigued before you start but I've proved I can turn my hand to any type of time trial."
Just how good his time was became apparent when Wiggins, the Olympic time trial champion, reached the first intermediate checkpoint at 26km 52 seconds adrift, although Wiggins had been forced to change his time trial bike after suffering a puncture.
Australia's Cadel Evans was 22 seconds slower than Dowsett and Ryder Hesjedal was 43 seconds back, although Nibali went six seconds quicker.
Wiggins, who rode cautiously to the finish after crashing in wet conditions on Friday, appeared to again take care on the descents in the time trial, while the likes of Nibali and Evans looked to be taking more risks, knowing they had to limit their losses to superior time triallist Wiggins.
The Team Sky rider coped better with the second half of the race, which included a three-and-a-half kilometre climb to the finish, and stopped the clock just 10 seconds behind Dowsett to gain 11 seconds on Nibali, 29 on Evans and more than two minutes on Hesjedal.
"I think there was some initial disappointment because I wanted to win the stage," Wiggins told Team Sky's website.
"It's been a challenging few days with the crash [on Friday]. It wasn't an easy course. I've said all along that it wasn't one of those ones where you could take three or four minutes out of people because it was so technical at the start.
"Then obviously to have a puncture and have to change bikes and disrupt the rhythm is never going to help. But then the second part of the course was really suited to me and I took back a lot of time on people there.
"It is what it is and it's put us right back up there now. It's going to be a hell of a race for the next two weeks."
Saturday's result means Italian Nibali leads Evans by 29 seconds overall, with Dutchman Robert Gesink moving into third, one second ahead of Wiggins, after a top-10 finish on the time trial.
"I wasn't expecting to be wearing the pink jersey today," conceded Nibali. "Now we will go about defending the pink jersey, I'm feeling comfortable."
Sunday's stage nine is a 170km race from Sansepolcro-Firenze which features a couple of tough climbs, including a 9km category one ride up to Vallombrosa.
Stage eight results
1. Alex Dowsett (GB/Movistar) 1:16:27"
2. Bradley Wiggins (GB/Team Sky) +10 secs
3. Tanel Kangert (Ita/Astana) +14 secs
4. Vincenzo Nibali (Ita/Astana) + 21 secs
5. Stef Clement (Ned/Blanco) +32 secs
General classification standings
1. Vincenzo Nibali (Ita/Astana) 29:46:57"
2. Cadel Evans (Aus/BMC Racing) +29"
3. Robert Gesink (Ned/Blanco) +1:15"
4. Bradley Wiggins (GB/Team Sky) +1:16"
5. Michele Scarponi (Ita/Lampre) + 1:24"
6. Ryder Hesjedal (Can/Garmin) + 2:05"