Glamorgan travel to Surrey on Sunday hoping to record three wins in three days.
Captain Mark Wallace does not think the tight schedule will affect his players.
"It's a lot easier to rock up and enjoy your cricket when you come off the back of a fantastic win," he said.
"The body's are fine."
Jim Allenby (70) and Jacques Rudolph (61) put on 136 for the first wicket for Glamorgan in the county's opening One-Day Cup match.
The Welsh county eased to their victory target with more than 13 overs to spare.
Earlier, Middlesex slumped to 90-8 with Michael Hogan (3-46) and David Lloyd (3-25) doing most of the damage.
"We were fantastic from the start," said Wallace.
"The way the two guys [Allenby and Rudolph] batted on what was a slightly difficult surface was fantastic so we are really happy with the win."
James Harris, on loan from Middlesex, is expected to start for Glamorgan against Surrey at Guilford.
The Welsh county are expected to rest Australian seamer Michael Hogan for the 50-over clash in Group B.