The US military says it is cancelling $300m (£230m) in aid to Pakistan over what it calls Islamabad's failure to take action against militant groups.
President Donald Trump has previously accused Pakistan of deceiving the US while receiving billions of dollars.
Pentagon spokesman Lt Col Koné Faulkner said the US military would aim to spend the money on other "urgent priorities".
The move, which needs to be approved by the US Congress, is part of a broader suspension announced in January.
The US state department has criticised Pakistan, a key ally, for failing to deal with terrorist networks operating on its soil, including the Haqqani network and the Afghan Taliban.
"We continue to press Pakistan to indiscriminately target all terrorist groups," Col Faulkner said in a statement on Saturday, adding that the $300m aid - which had earlier been suspended - should be used elsewhere due to "a lack of Pakistani decisive actions" in tackling the issue.
The announcement comes just days before US Secretary of State Mike Pompeo is due to visit Pakistan to meet the country's new prime minister, Imran Khan.
In January, the US government announced it was cutting almost all security aid to the country.
The US and others have long complained that Pakistan provides a safe haven to militant networks, allowing them to carry out cross-border attacks in Afghanistan - something that Islamabad denies.
There was no immediate comment from Pakistan on the latest funding cut.
However, the military responded to January's suspension by arguing that it "never fought for money but for peace", and had targeted all militants at a "heavy cost of blood and treasure".
Separately on Friday, the US said it was ending all funding for the UN's Palestinian refugee agency - the United Nations Relief and Works Agency (Unrwa) - which it described as "irredeemably flawed".
Who are the militants Pakistan is alleged to support?
The Haqqani network is a militant group that focuses most of its activities on neighbouring Afghanistan, which has complained for years that Pakistan allows it to operate unimpeded from its soil across the border.
The group is linked to the Afghan Taliban - a hardline Islamist movement that poses a major threat to the Afghan government. Pakistani Taliban groups, while associated with the Afghan Taliban, focus on attacks within Pakistan.
Both the Haqqani Network and the Afghan Taliban have launched attacks in Afghanistan that have killed US forces, and US officials have long argued that Pakistan, and specifically its ISI intelligence service, provides safe havens to them.
Why would Pakistan support them?
Pakistan has long been accused of using the Afghan Taliban to further its foreign policy interests in the country. The ISI first became involved in funding and training militants in Afghanistan after the Soviet invasion in 1979.
Although since 2001 Pakistan has allowed its territory to be used to supply international troops during the war in Afghanistan, and co-operated with the West in fighting some terrorists groups like al-Qaeda, analysts say it has continued to give shelter and support to Afghan insurgents.
Its aim has been to limit the influence in Afghanistan of its chief regional rival, India.