Greece has missed the deadline for a €1.5bn (£1.1bn) payment to the International Monetary Fund (IMF), hours after eurozone ministers refused to extend its bailout.
But the ministers say they will discuss a last-minute request from Greece for a new two-year bailout on Wednesday.
Greece is the first European Union country to fail to repay a loan to the IMF and is now formally in arrears.
There are fears that this could put Greece at risk of leaving the euro.
The IMF confirmed that Greece had failed to make the payment, shortly after 22:00 GMT on Tuesday.
"We have informed our Executive Board that Greece is now in arrears and can only receive IMF financing once the arrears are cleared," said IMF spokesman Gerry Rice.
Mr Rice confirmed the IMF had received a request from Greece to extend the payment deadline, which he said would go to the board "in due course".
With the eurozone bailout expired, Greece no longer has access to billions of euros in funds and could not meet its IMF repayment.
The European Central Bank (ECB) has also frozen its liquidity lifeline to Greek banks. Meanwhile, ratings agencies have further downgraded the country's debt.
Analysis: Chris Morris, Europe correspondent
This is not how international diplomacy or finance is supposed to be conducted: a series of confusing last minute proposals in an atmosphere of mounting chaos.
Without another bailout, Greece is in dire straits - cut off from all international financing, and skating on dangerously thin ice.
If it can't repay a debt to the ECB on 20 July, that would probably be the end. It is running out of options to keep it in the eurozone.
Part of the new deal Greece has suggested would involve a restructuring of its huge debts - but some of its proposals will not be acceptable to other eurozone countries. So there is uncertainty at every turn, and plenty of public posturing as Greece prepares for a referendum on Sunday.
Just to confuse matters still further, some EU officials have suggested that there have been indications from the Greek authorities that if the outlines of a deal can be done in the next couple of days, the referendum could even be cancelled.
New bailout 'considered'
Eurogroup chairman and Dutch Finance Minister Jeroen Dijsselbloem earlier said it would be "crazy" to extend the Greek bailout beyond its Tuesday deadline as Athens was refusing to accept the European proposals on the table.
Greece's left-wing Syriza government, elected on an anti-austerity platform, has been in deadlock with its creditors for months over the terms of a third bailout.
Speaking after the conference call with other eurozone ministers, Mr Dijsselbloem said that a Greek request for a new €29.1bn European aid programme would be considered in a telephone conference on Wednesday.
The call will take place at 15:30 GMT.
Greek newspaper headlines on Wednesday
Ta Nea (centre-left): "Fears of levy on deposits" - "The ghost of Maidan [Square in central Kiev, Ukraine] over Syntagma Square: The tension between pro-European and anti-European supporters is mounting as we approach Sunday's referendum, leading to fears of Ukraine-style division"
I Efimerida (left-of-centre): "'NO' ahead" - "But blackmail of bank closure is catalyst for people's decision"
Ethnos (centrist): "Zero hour" - "The country under siege since midnight"
I Avyi (pro-government): "'NO" a catalyst for a new deal
Rizospastis (Communist Party newspaper): "They are asking for a new 'bailout deal' noose for the people!"
Eleftheros Typos (right-of-centre): "Greece defaults and Tsipras plays" - "In confusion, the prime minister asks for a restructure of the bailout and a strict agreement"
BBC economics correspondent Andrew Walker says he understands Greece may submit new proposals on Wednesday that rein in its spending.
Greece's request on Tuesday asked for funds from Europe's bailout fund - the European Stability Mechanism - as well as a restructuring of Greece's public debt.
But German Chancellor Angela Merkel earlier said she had ruled out further negotiations until after Sunday's referendum, which will ask Greeks if they want to accept the deal offered by their creditors.
The Greek government took the unilateral decision to hold a vote last weekend, angering eurozone ministers.
What happens next?
1 July - Eurogroup - the finance ministers of the eurozone - holds a telephone conference to discuss new proposal from Greek Prime Minister Alexis Tsipras
5 July - Referendum on creditors' proposals takes place, which many say is effectively a vote on Greek membership of the eurzone
20 July - Greece must redeem €3.46bn of bonds held by the European Central Bank. If it fails to do so, the ECB can cut off Greece's access to emergency loans
Meanwhile, the US Treasury said on Tuesday it was urging all parties to press forward with negotiations "that put Greece on a path toward economic growth within the eurozone".
The European Commission - one of the "troika" of creditors along with the IMF and the eurozone's European Central Bank - wants Athens to raise taxes and cut welfare spending to meet its debt obligations.
Ratings agency Fitch cut Greece's credit rating from "CCC" to "CC" on Tuesday evening, which is one level above a full default.
Fears of a default on Greece's huge public debt of €323bn have led to long queues of people at cash machines. Withdrawals are capped at just €60 a day.
Greek banks did not open this week after talks between Greece and its creditors broke down.
However, up to 1,000 bank branches are re-opening on Wednesday to allow pensioners - many of whom do not use bank cards - a one-off weekly withdrawal of up to €120.
On Tuesday evening, thousands of pro-EU protesters braved stormy weather and gathered outside the Greek parliament in Athens to urge a 'Yes' vote in a referendum on Sunday over whether the country should accept its creditors' proposals.
It follows a similar demonstration by those advocating a 'No' vote - the path preferred by Mr Tsipras - on Monday.
EU leaders have warned that a 'No' vote rejecting creditors' proposals would mean Greece leaving the eurozone - though Mr Tsipras says he does not want this to happen.
Only three other countries are still in arrears to the IMF - Sudan, Somalia and Zimbabwe. Between them, they owe €1.6bn, only marginally more than Greece.
Lenders' proposals - key sticking points
- VAT (sales tax): A new system to come in from 1 July, with three rates, aimed at boosting annual revenue by 1% of total output (GDP)
- Most goods to be taxed at top rate of 23%, including restaurants and catering and processed foods
- Reduced rate of 13% for basic food, electricity, hotels and water
- Super-reduced rate of 6% for medicines, books and theatre
- End exemptions and eliminate VAT discounts for Greek islands
- Create strong disincentives to early retirement
- Move retirement age up to 67 by 2022
- End Ekas "solidarity" top-up grant that some 200,000 poorer pensioners get - immediate Ekas cut for wealthiest 20% of recipients, and cut completely by 2020
- Pensioners' healthcare contributions to rise to 6%, from 4%