Passengers faced delays and disruption during a 24-hour walkout by Tube drivers.
The strike on Central and Waterloo & City lines ended at 21:00 GMT on Wednesday. Services are not expected to return to normal until Thursday.
Rail and Maritime Transport (RMT) members were protesting about relocation plans. Tube bosses said the strike was "unnecessary".
That dispute centres on a long-running row over the role of train guards.
The Tube dispute involves a plan to move eight train operators from an east London depot to one in the west of the city.
The RMT reiterated calls for London Underground to "take the issues at the heart of the dispute seriously".
Aslef union members have also taken part in the strike.
Peter McNaught, operations director for the Central Line, apologised for the disruption.
He added: "We have made all reasonable efforts to resolve this dispute through talking."
RMT general secretary Mick Cash said the "door has been slammed in our faces", despite "strenuous efforts" to resolve the dispute.
"If London Underground is allowed to get away with this move on the Central Line they will start shunting drivers around at the drop of a hat, regardless of the consequences," he added.
Maintenance workers on London Underground have separately voted to strike over a breakdown in relations. No date has been set for action.
Members voted to strike by two to one, and by nine to one for other forms of industrial action.
The RMT is now considering what form its action should take.