Network Rail is to build a £25m national road haulage distribution centre on a former car plant site in Warwickshire, the company has said.
It said the centre at Ryton would create new jobs, but it also planned to relocate workers from depots in Lichfield, Worcester and Ludgershall.
Those previously leased depots would close to "cut costs", a spokesman said.
The site at Ryton has been empty since the Peugeot car plant there was closed and demolished in 2007.
Network Rail said the workers affected by the move included a number of its own employees and contractors.
It said no Network Rail jobs would be lost as part of the relocation to Ryton.
The 300,000 sq ft (28,000 sq m) centre will have access to major main lines and to the A45 and motorway network.
Martin Elwood, from Network Rail, said the company's "first preference" was to transport goods by rail but this was "not always the right solution".
He said: "We transport millions of tonnes of railway products by rail - sleepers, ballast, that sort of thing - and that constitutes for about 80% of what we do.
"This depot is for the other 20%, the 10,000 small parts ranging from lightbulbs to toilet paper which are delivered to 360 other stocking points around the country."
The company hopes to cut its carbon footprint by reducing the mileage of the 8,000 road vehicles it operates.
The new depot is expected to open in July 2013.