A second woman in her 80s has died following a minibus crash that left 18 other people injured.
The bus overturned in the collision, also involving a car, near Bluntisham in Cambridgeshire, on Thursday.
Bus passengers Margaret Henwood, 85, of Foxlease, Bedford, died at the scene and Barbara McGruer, 86, of Trent Road, Bedford, died in hospital on Saturday.
Another passenger is in a critical condition and a further 10 people remain in hospital.
All of those sustained serious injuries, including the driver of the car - a man in his 70s, police said.
Eighteen of the minibus passengers, all aged between 60 and 80, were taken to hospital following the crash on the B1040 Somersham Road at 16:51 GMT.
Police said the group on the minibus was travelling home after a visit to Ely Cathedral's Christmas fair.
The ambulance service sent 10 ambulances, a rapid response vehicle, the Hazardous Area Response Team and two air ambulances to the scene.
Since Thursday's accident, another crash has occurred at the same junction.
Two cars and a van were involved at the B1040 crossroads at about 15:20 GMT on Monday. Police say no injuries have been reported. Officers are still at the scene and the road remains open.
More than 6,000 people have signed a petition which was set up after Thursday's crash, calling for improvements to be made at the junction.