Tens of thousands of motorcyclists are arriving in Hastings for the 35th May Day festival, which attracts riders from across London and the South East.
Bike1066 spokesman Howard Martin said fine Bank Holiday weather could attract up to 100,000 bikers and spectators to the East Sussex town.
"It shows how important motorcycling is as a leisure lifestyle of high value to towns like Hastings," he said.
"It is a fantastic phenomenon and everybody is welcome to come and see."
Road safety campaign
Hastings has been a destination for bikers since the mods and rockers influx of 1964.
The May Day run originally started at Locksbottom on the Kent border with south London and followed the A21 to Hastings sea front.
Now some 32,000 bikers also meet at places such as the Ace Cafe in north London and Box Hill in Surrey.
Sussex Police are using the event to warn motorcyclists against riding when they are tired.
The force is supporting Mike's Last Ride, a road safety campaign launched by Insp Dave Mangan, of Lancashire Police, whose father died after being involved in a motorbike accident while tired.
"We always tell car and lorry drivers to stop to have a break if they feel tired on the roads," said Sgt Dan Pitcher.
"Bikers need to take that advice too as if they lose concentration it is even easier for them to put themselves in real danger."
Mr Martin said the campaign video would be shown at the East Sussex Fire and Rescue stand in Pelham car park.
"It's not just a warning to bikers - it's a warning to all road users to be aware of their surroundings, check their fatigue and take a break," he said.