James Charles has lost more than one million subscribers amid a row with fellow YouTube star Tati Westbrook.
He now has 14.2m subscribers - previously the figure was 16m, according to the website Social Blade.
It comes in a week where the 19-year-old has denied using his fame and money to try to manipulate someone's sexuality.
Meanwhile, his row with Westbrook centres around him promoting a rival product to one she'd created.
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Westbrook and Charles have both amassed enormous social media followings through make-up tutorials and beauty product reviews.
He says the 37-year-old took on a "parental" role after they met three years ago.
However in a video titled BYE SISTER, Westbrook said she was cutting all ties with him.
"When you paired up with my number one competitor it was embarrassing for me and you know that.
"You kept saying you can't be bought, but you just were," she said.
As well as accusing him of being disloyal to their friendship, Westbrook also claimed Charles tried to "manipulate" people's sexuality.
She said the teenager would regularly boast about wanting to turn straight men gay.
She said: "You are using your fame, power and money to play with people's emotions.
"I don't want to be friends with you. I don't want to be associated with you."
More than 23 million people have watched the video since it was uploaded on 10 May.
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So blessed to have this amazing soul in my life ❤️ @jamescharles Thank you for helping me feel so beautiful on such an important day ... your talent is out of this world, but you as a person are even more impressive & special. I'm keeping you close as a friend forever. Love you so much Sister 😘😘😘 Photo Credit: @kaua_wedding_photography
Following the release of Westbrook's video, Charles posted a video on his own channel.
Simply called Tati, he apologised for "everything that I put you through".
"What sucks the most is that I know there's nothing I can say or do to ever earn that friendship or trust back, but I don't blame them for it.
"A lot of the time when I've had to address things in the past, I've acted out of impulse and I've gone off and tried to pull receipts or facts or screenshots and play the victim.
"I'm not doing that today, I'm not. That is all I have to say, I'm sorry."
The 19-year-old however denied he'd ever pressured someone into dating him but conceded he'd perhaps mishandled situations in the past.
He said: "I've been involved in a lot of very unique situations that have left people confused or upset.
"I have learned the hard way about boys that I am interested in, and also ones that I should or shouldn't be talking to.
"This is a conversation I know a lot of people are uncomfortable with and it's something that I should have been far more careful with bringing into the public, especially with the last situation," Charles said in the video.
Charles' comments via his video statement could be in relation to a tweet he posted.
In it, he denied starting a romantic relationship with a man who was "unsure" about his sexuality.
Charles, who is worth an estimated $12m (£9m), has collaborated with celebrities including Katy Perry, Kim Kardashian and Demi Lovato.
In 2016, he made history when at 17 he became the first ever male to pose for beauty brand CoverGirl.