Eight Labour MPs have tabled a series of motions in the Commons urging London Underground to rethink plans to reduce the opening times of ticket offices.
The loss of 800 ticket office and gate line jobs would "adversely" affect Tube services, the MPs said.
Disabled people may find it difficult to travel and passengers would avoid travelling too early or late at night due to safety concerns, they added.
Transport for London said stations will be staffed while trains operate.
In March London Underground announced that up to 800 jobs - including 100 managers, 450 ticket office posts and 200 other jobs - will be axed under plans to make savings of £16m a year.
The early day motions were tabled by four London MPs - Glenda Jackson for Hampstead and Kilburn, MP for Hayes and Harlington John McDonnell, Jeremy Corbyn for Islington North and MP for Ealing Southall Virendra Sharma.
The four other MPs were - MP for Glasgow South West Ian Davidson, MP for Blythe Valley Ronnie Campbell, Dundee West MP Jim McGovern and Halifax MP Linda Riordan.
They said: "Transport for London's proposals to shed up to 800 ticket office and gate line jobs on London Underground will adversely affect the opening times of ticket offices."
Customer service will also be hit with passengers "forced to queue for longer to buy their tickets" and that machines were "no replacement" for trained staff, they claim.
They highlighted ticket offices in 14 stations in north and west London - Kilburn Park, Kensal Green, Queen's Park, Finchley Road, Swiss Cottage, West Hampstead, Belsize Park, Hampstead, Tufnell Park, Archway, Arsenal, Finsbury Park, Northfields and South Ealing.
A TfL spokeswoman said: "We have assured staff and customers that all of our stations will continue to be staffed at all times while trains are operating, and that all stations with a ticket office will continue to have one.
"The changes we're proposing to ticket office opening hours are in line with customer demand."