Steve Rider has quit his role as a presenter on ITV Sport by "amicable agreement", the network has announced.
His departure comes shortly after the appointment of Adrian Chiles to front the channel's football coverage, including the World Cup.
ITV Sport controller Niall Sloane said he hoped to continue to work with Rider on different projects in the future.
Rider first joined ITV Sport 33 years ago, but was a well-known face on BBC Sport from 1985-2005.
He returned to ITV that year to front coverage of Formula One racing, adding football to his duties the following year.
Rider began his career in the 1980s at ITV as a regular host of World of Sport and presenting the station's coverage of the 1980 Moscow Olympic Games.
Mr Sloane said: "Steve has presented a huge range of sports programming during his time at ITV, and some of the most watched broadcasts of recent years."
Rider added: "It was important to get this sorted before such an important few weeks for ITV Sport. I wish Adrian all the best for what lies ahead."
Chiles, former presenter of The One Show, quit the BBC to front ITV's football coverage and host GMTV five mornings a week.
His departure came after the decision to bring in Chris Evans to host the BBC One teatime show one day a week.