The metabolism of a micro-organism can be controlled genetically and can involve mutagenesis and recombinant DNA technology.
Mutagenic agents can cause mutagenesis. Which of the following is a chemical mutagenic agent?
What is the preferable vector when larger fragments of DNA are required to be inserted?
DNA can be cut into fragments. Which group of enzymes can do this?
What name is given to the fragment of DNA shown?
What name is given to the enzyme that joins two fragments of DNA together?
What is the vector shown in this diagram?
Which type of cells can be used to produce active proteins that are normally inactive in bacteria?
Recombinant yeast cells
Recombinant bacterial cells
Recombinant archaea cells
Recombinant plasmids and artificial chromosomes contain the following:
Restriction sites, regulatory sequences, an origin of replication and selectable markers
Regulatory sites, regulatory sequences and an origin of replication
Regulatory sites and regulatory sequences
Selectable marker genes do what?
Ensure that only the mirco-organism that have taken up the vector grow in the presence of a selective agent (antibiotic)
Ensure that only the micro-organism that have not taken up the vector grow in the presence of a selective agent (antibiotic)
Have no effect on the micro-organism
As a safety mechanism what is often introduced that prevents the survival of a micro-organism in an external environment?