Preparing biological samples for examination

Aims of the experiment

  • To stain cells for examination with a light microscope.
  • To examine a range of cells and other structures with a microscope to understand their basic structure.
biological samples for examination

Animal cells

Cheek cells

Completing the microscope slide for examination

When viewing any slide with a microscope, a small square or circle of thin glass called a coverslip is placed over the specimen. It protects the microscope and prevents the slide from drying out when it's being examined.

The coverslip is lowered gently onto the specimen. A mounted needle can be used to hold the specimen in place as the coverslip is lowered. It is important that no air bubbles are trapped underneath.

Staining the cells

Most cells are colourless. Stains are used to add contrast. Certain stains are also used to stain specific cell structures or cell products.

Step by step process of staining cells for examination.

Risks

  • Care must be taken when looking down the microscope if the illumination is too bright.
  • Care must be taken when using microscope stains.
  • Care must be taken when handling coverslips, microscope slides and mounted needles.