Practical - Anaerobic respiration

Anaerobic respiration can be demonstrated by removing oxygen from yeast’s surroundings.

Anaerobic respiration yeast


  1. Boil the glucose solution to sterilise it and remove any oxygen, leaving behind the glucose needed for anaerobic respiration.
  2. Cool before adding the yeast (high temperatures will kill it).
  3. Place a layer of oil on top of the glucose solution to prevent oxygen entering.
  4. Yeast will respire anaerobically producing alcohol, heat and carbon dioxide that can be collected in limewater.

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