Extracting DNA from fruit

It is possible to extract DNA from cells in a variety of ways. One of the simplest methods is to extract it from fruit like a kiwi.

Aim

The aim of this experiment is to extract DNA from a fruit.

Method

  1. Peel the skin from half a kiwi fruit and mash it up
  2. Mix a teaspoon of salt and small volume of washing up liquid into the fruit
  3. Gently heat this mixture at about 60°C for five minutes
  4. Filter the mixture and retain only the filtrate (the filtered liquid)
  5. Cool using an ice bath and gently pour chilled ethanol onto the top of the filtrate

You will see strands of DNA with bubbles in them at the boundary between the filtrate and the chilled ethanol.

The important aspect of this practical is the method you would use. This experiment is not like other practicals in that the results cannot really be evaluated.

Filtering a mixture during a DNA extraction experiment
Filtering a mixture during a DNA extraction experiment
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