On 6 May 1565, Mary sought permission to marry Darnley, and on 15 May, this was granted by a meeting of the nobility.
Mary and Lord Darnley met in February 1565 at Wemyss Castle in Fife and were married at Holyrood Palace in July the same year.
The marriage was to cause divisions amongst the Lords.
With Elizabeth protesting strongly against it, Moray, Argyll and Chatelherault agreed to rebel in March 1565. On 14 August the Crown seized the lands of the rebel Lords, who had mobilised against Mary near Ayr but with little support.
With strong support and an army of 5000 men, Mary left Glasgow determined to pursue the rebels.
The rebel Lords asked Elizabeth for help. She refused to give it as it may set a precedent for nobles within England to turn against their monarch. However, a number of nobles sought refuge in England and Mary lost the support of strong and powerful men, among them the Earl of Moray.
It took until October to restore calm and Mary’s victory proved to be a damaging blow to the Protestants.
Yet, Mary had married a man of her choice. She had defied Scottish nobles and Queen Elizabeth, but kept the support of France, Spain and the Vatican. For a short time at least, her people remained loyal.