With Elizabeth protesting strongly against the marriage, Moray, who was strongly opposed to Henry Stuart’s family, decided to rebel against Mary. He was joined by Argyll and Chatelherault in March 1565:
With strong support and an army of 5000 men, Mary left Glasgow determined to pursue the rebels. On 14 August the Crown seized the lands of the rebel Lords who had mobilised against Mary near Ayr.
The rebels meanwhile reached Edinburgh. However, there was little support for them there and as a result they could not seize power. At this point, they waited in Dumfries for Elizabeth I to send help. She refused to give it as it may have set a precedent for nobles within England to turn against their monarch:
It took until October to restore calm and Mary’s victory proved to be a damaging blow to the Protestants. A number of nobles sought refuge in England and Mary lost the support of strong and powerful men, among them the Earl of Moray.
Yet, Mary had married a man of her choice. She had defied Scottish nobles and Queen Elizabeth, but kept the support of France, Spain and the Vatican. For a short time at least, her people remained loyal.