It is more than 11 years since Williams made his Test debut, against France in the opening match of the 2000 Six Nations.
A try tribute to Shane Williams
In that time the Ospreys star has scored a Wales record 57 tries, played in three World Cup campaigns and toured twice with the British and Irish Lions.
Wales coach Warren Gatland paid tribute to the
Amman Valley product
: "He will leave a huge hole, he is our record try-scorer.
"It's a great opportunity on Saturday for someone to determine his own destiny and say 'this is my last Test'.
"It gives us a chance to give him a send-off as a team and for the Welsh public to see him play at the Millennium Stadium for the last time.
"It will be fantastic for him to lead the team out and get the standing ovation he deserves, and for the boys to do his last game for Wales justice by playing well.
"We know Australia will be tough this weekend. In the game against the Barbarians [last Saturday] they made a very slow start, but as game went on they looked very strong."
Leigh Halfpenny (Cardiff Blues); George North (Scarlets), Scott Williams (Scarlets), Jamie Roberts (Cardiff Blues), Shane Williams (Ospreys); Rhys Priestland (Scarlets), Lloyd Williams (Blues); Gethin Jenkins (Blues), Huw Bennett (Ospreys), Scott Andrews (Blues), Bradley Davies (Blues), Ian Evans (Ospreys), Dan Lydiate (Newport Gwent Dragons), Sam Warburton (Blues, capt), Toby Faletau (Dragons).
Matthew Rees (Scarlets), Ryan Bevington (Ospreys), Ryan Jones (Ospreys), Justin Tipuric (Ospreys), Tavis Knoyle (Scarlets), Dan Biggar (Ospreys), Alex Cuthbert (Blues).
Adam Ashley-Cooper (NSW Waratahs); Lachie Turner (NSW Waratahs), Anthony Fainga'a (Queensland Reds), Berrick Barnes (NSW Waratahs), Digby Ioane (Queensland Reds); James O'Connor (Melbourne Rebels), Will Genia (Queensland Reds); James Slipper (Queensland Reds), Tatafu Polota Nau (NSW Waratahs), Salesi Ma'afu (Western Force), James Horwill (Queensland Reds, capt), Rob Simmons (Queensland Reds), Scott Higginbotham (Queensland Reds), David Pocock (Western Force), Ben McCalman (Western Force).
Stephen Moore (Brumbies), Ben Alexander (Brumbies), Nathan Sharpe (Western Force), Radike Samo (Queensland Reds), Ben Lucas (Queensland Reds), Ben Tapuai (Queensland Reds), Nick Phipps (Melbourne Rebels).
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