Bolt, who won both the 100m and 200m at the Beijing Games in 2008 and is the world record holder in both events, finished second in 9.86 secs, with former world record holder Asafa Powell third in 9.88.
"Definitely I am on the right path for the Olympics," said Blake after Tuesday's victory. "I didn't come here to run a quick time but it's still a fast time."
Blake and Bolt, who are training partners, will be the favourites for 100m and 200m gold in London when the Games start later this month. They are the only sprinters to run faster than 9.80 seconds this season.
American Justin Gatlin, who won gold at the Athens Games in 2004, has clocked 9.80 this year, while Asafa Powell, another Jamaican, has run 9.85, with Trinidad and Tobago's Keston Bledman posting 9.86.
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