Frankel's team are expecting their unbeaten charge to face a field determined to ruin what is likely to be his final ever race later this month.
The four-year-old pleased the Newmarket crowds with a workout on Saturday ahead of Ascot's Champion Stakes.
Frankel's lucky 13
13 August 2010:
European Breeders' Fund Maiden Stakes, Newmarket
10 September 2010:
Frank Whittle Partnership Condition Stakes, Doncaster
25 September 2010:
Juddmonte Royal Lodge Stakes, Ascot
16 October 2010:
Dubai Dewhurst Stakes, Newmarket
16 April 2011:
Greenham Stakes, Newbury
30 April 2011:
2000 Guineas, Newmarket
14 June 2011:
St James's Palace Stakes, Ascot
17 July 2011:
Sussex Stakes, Goodwood
15 October 2011:
Queen Elizabeth II Stakes, Ascot
19 May 2012:
JLT Lockinge Stakes, Newbury
19 June 2012:
Queen Anne Stakes, Ascot
1 August 2012:
Sussex Stakes, Goodwood
22 August 2012:
Juddmonte International Stakes, York
And Teddy Grimthorpe, race manager for owner Prince Khalid Abdullah, told BBC Look East: "Every race he goes to he's got to be on top form.
"Everyone wants to have a shot at the champ," he added.
Frankel, trained at Sir Henry Cecil's Warren Place stables in Newmarket,
has won all 13 of his races
will look to make it 14 at Ascot,
before the word's top-rated horse is expected to be retired to stud.
"You never say never but I think in everyone's minds this will probably be his last race," said Grimthorpe.
"We've had three wonderful years. He's been exceptional literally from day one. He's raised the bar a little bit I'd say.
Grimthorpe expects Frankel to retire
"He's given everyone really tremendous times particularly Prince Khallid and everyone at Warren Place."
Saturday's workout before the Cambridgeshire Day card saw Frankel gallop with stable companions Specific Gravity and his half-brother Bullet Train over nine furlongs.
Ridden by regular jockey Tom Queally,
Frankel looked strong and relaxed over the nine furlongs on the Rowey Mile.
"We want him there in the best possible shape [for Ascot]," said Grimthorpe.
"We learnt that he's in good form. Or he still is in good form. He's moving very nicely, which is most important. All the right signs are there.
"With a horse of his calibre the expectations are enormous. But the things that have to go right for Frankel are still the same.
"He's got to be healthy, sound and in a good frame of mind. And the race has got to go pretty much to plan. There are glorious uncertainties in racing which always make it interesting."