Canford Cliffs also won the St James's Palace Stakes at Royal Ascot last year
Richard Hannon has his eye on potential clashes with Goldikova and Frankel later this season after Canford Cliffs made a successful reappearance in the Lockinge Stakes at Newbury.
Canford Cliffs was unspectacular in beating Italian raider Worthadd.
Hannon and jockey Richard Hughes expect their colt, a 4-5 favourite on Saturday, to be in better shape in time for his next challenge.
That is expected to come against Goldikova in the Queen Anne Stakes.
Worthadd led for most of the race when all seven jockeys appeared reluctant to show their hand and it was only inside the final furlong that a 25-1 upset was not realised.
"He hadn't run for a long time and he'll come from that," said Hannon following Canford Cliffs' length-and-a-quarter success.
"He's done everything very well but you look at him and think, 'Oh, he's a little bit burly'.
"Ascot will be the next port of call now. We don't mind taking on Goldikova, she's getting old, and Frankel too - bring them on.
"You can run but you can't hide and, as far I'm concerned, he's going to be there to take on all-comers.
"Frankel won't be able to do what he did to the 2000 Guineas field to him.
"Canford Cliffs has won four Group Ones now so he'll be a real stallion - I'd love to have him next season as a five-year-old, but we'll see. He's the best horse I've ever had."
Twice Over wilted to finish sixth when initially ridden up with the pace, while Premio Loco claimed third, ahead of Canford Cliffs' stablemate Dick Turpin.