Scottish Cup newcomers Formantine United and Turriff United both progressed to the second round.
Highland League outfit Turriff hammered Kingdom Caledonian League side Burntisland Shipyard 6-1.
And Formantine edged out Brora Rangers 3-2 in an all-Highland tie.
Junior Cup winners Shotts Bon Accord were held to a 1-1 draw at home to East of Scotland League outfit Edinburgh City, fellow juniors Bonnyrigg Rose and Irvine Meadow won but Hermes lost.
East Superleague champions Bonnyrigg defeat Ayrshire juniors 2-1, while West Superleague champions Meadow eased aside East of Scotland side Gala Fairydean 4-0.
But North Superleague champions Hermes were swept aside 4-1 at home to Highland League side Deveronvale.
St Cuthbert Wanderers, who defeated Highland outfit Keith in a preliminary round tie, won again, defeating East of Scotland outfit Edinburgh University 2-1 in the capital.
Another South of Scotland League side, Wigtown & Bladnoch, surprised Huntly, taking the Highland League side back to their own patch after a 2-2 draw.
The all-Highland League clash between Buckie Thistle and Rothes will also go to a replay after a stalemate at Victoria Park.
Also progressing from the Highland League were Clachnacuddin, Fraserburgh, Golspie Sutherland, Nairn County, Wick Academy and Inverurie Loco Works.
There were also wins for Civil Service Strollers, Selkirk and Vale of Leithen of the East of Scotland League.
Scottish Cup first round results:
Bonnyrigg Rose 2-1 Girvan
Buckie Thistle 0-0 Rothes
Civil Service Strollers 4-0 Newton Stewart
Clachnacuddin 2-1 Lossiemouth
Edinburgh Univiversity 1-2 St Cuthbert Wanderers
Formartine United 3-2 Brora Rangers
Fraserburgh 4-0 Colstream
Glasgow Univiversity 0-2 Selkirk
Hawick Royal Albert 2-4 Golspie Sutherland
Hermes 1-4 Deveronvale
Huntly 2-2 Wigtown & Bladnoch
Irvine Meadow 4-0 Gala Fairydean
Preston Athletic 0-2 Nairn County
Shotts Bon Accord 1-1 Edinburgh City
Spartans 0-2 Wick Academy
Threave Rovers 0-1 Vale Of Leithen
Turriff United 6-1 Burntisland Shipyard
Whitehill Welfare 2-4 Inverurie Loco Works