Scotland and EU: Michael Moore on legal advice
7 December 2012 Last updated at 14:21 GMT
The Scottish Secretary said the "most likely scenario" of an independent Scotland would see the rest of the UK remaining in the EU and the new nation "would have to apply to join the EU again".
Michael Moore said it raised a "huge set of issues" including fishing and banking, plus questions over joining the euro - as all new EU nations are compelled to work towards membership - and the Schengen agreement over border controls, which do not apply to the UK.
He was speaking after reports in the Scotsman newspaper of a letter from the European Commission, despite the Scottish government saying it would not have to re-apply.