Frank is the eldest and the thinker of the two brothers. He is active, busy and practical. He is a bit serious and doesn't often show his emotions. Frank likes to be seen as capable and dignified, so he is a bit vulnerable when things don't go his way. Frank acts as pilot when the two brothers go out on daily patrol. He's always in charge of the take-off routine and is usually the one who comes up with the perfect idea to solve a problem. He has a distinctive 'hands on hips' pose.
Buster is a gentle soul who wears his heart on his sleeve. He is innocent, open, instinctive and affectionate. He has a distinctive 'shrug' that sums up his calm, easy-going nature. He has a tendency to express his overwhelming affection for others with a direct and generous hug round the shoulders. Buster sits in the back of the plane with his telescope and acts as 'look-out' when the two brothers go out on their daily patrol.
Ned, the little wombat, lives in a caravan in the yard of the Koala Brothers' Homestead. He is the youngest of all our characters in the Outback. Ned admires the Koala Brothers (particularly Frank), and regards himself as an important member of the Koala Brothers' team. Ned always opens the gate and guides the plane out of the Homestead and will often salute Frank when given an instruction. He is often seen hitching up his trousers which are always working their way down! He loves to hopscotch and often has ambitions to 'be someone', like a fireman, policeman or sea captain.
Mitzi is a feisty little possum who lives in an extension at the side of the Koala Brothers' Homestead. She is a little powerhouse of energy, fast talking and confident, who is always on her way somewhere.
Archie the crocodile lives in a little house by the Waterhole. He's a good-humoured, sporty, sociable type who enjoys jogging and all other leisure pursuits. His big personality means that he is the life and soul of any party.
George the postman runs the local post office in town. He is rather slow at making his deliveries, by virtue of being a turtle, and he always appears a little out of breath as he has to travel long distances in the Outback to deliver the mail.
Sammy the echidna is the shopkeeper at the general store in town. Sammy likes to broaden his horizons and often sends away for information on a new hobby or pastime. Sammy is the only character who habitually uses the Australian greeting 'G'day!' whenever he appears with the groceries from his store.
Alice the platypus lives in town. She is a gentle soul who is good-hearted, kind and thoughtful. She rides a bright green scooter very carefully making all the correct hand signals even though she is in the middle of the Outback where you seldom see another vehicle.
Josie the little kangaroo helps out at the general store and mans the petrol pump in town whenever Sammy (the shopkeeper) is out on his errands. She is very shy, quiet and sweet-natured. Despite her shyness, Josie has hidden talents which often come to the fore.
Penny the little penguin is a backpacker who appears in various episodes as a visitor to the Outback. Penny is very sweet and appears to be an innocent abroad, but she is actually very capable. When she's happy she jumps up and down and claps her flippers - when she's sad she droops her head.
Lolly the emu serves the best ice-cream in the Outback! She runs the ice-cream van, which seems to appear at certain moments almost mysteriously out of nowhere, only announced by its tinkly tune. We mostly only see Lolly's head and long neck which cranes out of the serving hatch.